The Exercise and Performance Nutrition Laboratory has 快速ly evolved into an extremely well-equipped teaching and research laboratory. Many different forms of technology are available in the EPNL that are used as part of ongoing research and as part of classes within the Exercise Science program. The information provided below outlines the equipment and associated techniques available to our students that can be incorporated into various research studies and partnerships.
有氧能力
测量有氧能力通常被称为VO2 评估. 这种形式的评估被认为是确定一个人有氧健身水平的黄金标准. 在EPNL中使用代谢车测量有氧能力, 通常是在受试者走路的时候, 慢跑和骑自行车. 进一步评估健康状况, EPNL researchers can collect blood samples to measure changes in markers of energy metabolism and can also attach electrodes to the participants’ chest to assess their heart function.
无氧容量(Wingate)
无氧运动的特点是任何形式的快速运动, 快速, 爆炸性和非常高的强度. 测量无氧能力可以让研究人员了解一个人进行这种运动的能力. 许多类型的运动测试可用于评估一个人进行无氧运动的能力. 在EPNL中,厌氧能力通常使用Wingate测试进行评估. This cycle-based test is 30 seconds in duration and requires a participant to pedal as fast as they can against a resistance that is standardized to their body weight. 根据这次测试的结果, 峰值和平均发电量以及疲劳的速度都可以被评估.
生物化学功能
The EPNL also houses a small biochemistry laboratory that has the capability to 过程 and store nearly any biospecimen. 配备多台离心机, 分光光度计, 超低温冷冻机, 移液器和其他设备, EPNL研究团队在分析各种目标生物标志物之前,定期收集和处理样本.
虽然EPNL的分析能力正在迅速扩大, 该实验室目前拥有评估葡萄糖和乳酸盐等各种代谢物变化的设备, 血红蛋白和红细胞压积, 激素,比如生长激素, 胰岛素和睾酮, 细胞因子, 以及其他细胞靶标. 在我们的研究网络中建立了宝贵的合作伙伴关系, EPNL可以收集, 过程, store and analyze nearly any important biomarker commonly used as part of exercise and nutrition research studies.
Biospecimen集合
在EPNL完成的研究中,各种组织的采样是一个共同的特征. 用手指棒取样血液中葡萄糖的变化, 糖化血红蛋白, 胆固醇和乳酸都很容易完成,也很容易评估. Many studies require the sampling of venous blood using single stick venipuncture or multiple sampling techniques involving the placement of a catheter. 另外, 尿液取样可用于评估蛋白质代谢或各种营养物质的保留. 最后, EPNL的研究人员也接受过骨骼肌样本的训练和装备, 通常是从大腿开始, 作为各种研究技术的一部分.
身体成分评估
双能x射线吸收仪
来评估脂肪的变化, 无脂肪和骨组织作为各种运动和营养评估和干预的一部分, EPNL有一个Hologic QDR Discovery a DEXA. 这种设备使用x射线扫描整个身体并测量骨骼的数量, 手臂上的肌肉和脂肪, 腿和躯干. 除了身体成分评估, 这种装置也可以用来测量脊柱的骨密度, 髋部和前臂. 下面是使用DEXA的图片以及简短的视频.
生物电阻抗谱(BIS)
评估脂肪量的变化, 不含脂肪的质量, 肌肉, 身体的水, EPNL使用了一种受阻阻SFB7生物电阻抗谱装置. This piece of equipment requires appropriate hydration prior to testing and passes a low-level electrical current through electrodes in contact with the person’s hands and feet.
这个信号能够准确可靠地计算出一个人的体重, 身体成分, 和身体水分,同时适当地跟踪随时间的变化. We commonly use this device as part of our community testing and lecture seminars as well as our research studies. 准备考试大约需要10分钟,完成考试大约需要5分钟.生物电阻抗分析(BIA)
评估瘦体重的变化, 脂肪量, 体内总水量, an InBody 570 bioelectrical impedance analyzer (BIA) is used as a non-invasive technique used to assess 身体成分. BIA measures the impedance or resistance of body tissues to an electrical current that is passed through the body. It can be used to track changes in 身体成分 over time and evaluate the effectiveness of weight loss or exercise interventions. InBody 570是一种安全且易于使用的评估身体成分的工具, 它通常用于临床和研究环境.
心血管评估
EPNL有几种评估心血管属性的方法. 学生 and researchers in the EPNL can use 12-lead ECG to assess heart function at rest and in response to various forms of exercise. 除了, the EPNL has the capability to assess heart rate responses to exercise as well as heart rate variability over a 24-hour period using several types of heart rate monitors. These devices are used regularly as part of teaching and research protocols to appropriately measure changes in heart function in response to exercise and other research interventions.
认知评估
我们在EPNL中使用各种认知测试来评估研究中的认知功能. 这些测试包括NIH工具箱认知电池, Stroop颜色单词测试, 还有造径测试等等. 美国国立卫生研究院工具箱认知电池是一套全面的测试,旨在评估认知能力, 包括注意, 工作记忆, 处理速度, 以及执行功能. The Stroop Color Word Test is a classic test that assesses cognitive flexibility and inhibitory control while the Trail Making Tests A and B assess visual-motor speed and task switching ability. 我们的实验室也有Dynavision D2, 一个有64个3D目标的交互式光板, 这可以用来评估和训练认知和执行功能的各个方面, 包括反应时间和决策能力.
这些测试可用于评估研究中的认知功能, 临床的设置, 教育环境. 它们可以帮助识别认知缺陷,并追踪认知功能随时间的变化. 通过认知测试在我们的研究中的应用, we gain insights into the underlying mechanisms of cognitive function and identify potential interventions to enhance cognitive health. 整体, 认知测试是了解认知功能、促进健康老龄化的重要工具.
能量消耗 & 新陈代谢
Assessing changes in resting metabolism and how many calories are burned throughout exercise are extremely important measures for many of the research studies completed in the EPNL. 为了衡量这些结果, the EPNL has two ParvoMedics metabolic carts (pictured below) with the capability of quantifying energy expenditure during exercise as well as at rest. Many studies published by researchers in the EPNL have assessed changes and differences in resting metabolism over the course of entire athletic seasons, between different types of athletes and in response to exercise and nutrition interventions completed in recent research studies.
粪便收集和微生物组分析
EPNL staff is also trained at collecting and preserving freshly collected stool samples as part of various clinical investigations. 最近的项目包括分析,包括RNA后期保存, OmniGene保存, 和Whatman论文评价. 样品常规送到其他实验室进行散弹枪代谢组学和代谢组学分析.
肌肉健康(力量、力量和耐力)
EPNL有几种技术可以用来评估力量、力量和耐力. Many of these techniques are taught as part of classroom instruction and are regularly incorporated into research projects completed by students and faculty. EPNL拥有一个大的功率机架,可以确定一次重复最大值(1RM)。, 对一个人力量的直接衡量. 配合1RM测量, the EPNL regularly uses TENDO power analyzers which have a lanyard that connects to the end of the bar that measures how 快速ly the bar is moving during a weight lifting movement, 哪些可以用来评估动力和速度发展的变化.
除了1RM, the EPNL has a Biodex isokinetic dynamometer that allows students and researchers to assess static and dynamic force and power production (see video), the rate at which fatigue is developed in various muscles and the presence of any muscle imbalances across a joint.
最后, the EPNL has load-cells that can be interfaced to a computer that are used as part of isometric 评估s to measure strength. 这些设备提供的速度非常快, 可靠和敏感的评估力量生产的格式,是100%便携式. 这使得EPNL团队成员可以在实验室之外收集高质量的强度数据.
肺功能检查/肺量测定
Assessments of pulmonary function are important parameters that are used to detect various respiratory disorders such as COPD and asthma. 除了在实践实验课上使用外, these techniques are implemented as part of research studies to assess pulmonary function both at rest and after acute bouts of exercise.
超声波
超声肌肉形态学是一种用于评估人体肌肉结构和组成的方法. 该技术捕获目标肌肉的高质量图像,并评估肌肉厚度等特征, 夹角(肌纤维夹角), 肌肉纤维的长度. 测试前, 建议休息5分钟,以确保体液的适当重新分配. 这项技术对识别肌肉异常非常有用, 监控随时间的变化, 评估运动干预的有效性. 超声肌肉形态是评估肌肉健康和功能的一种无创和可靠的工具, 使其成为讲座、研讨会和研究的宝贵补充.